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 Table of Contents  
Year : 2020  |  Volume : 11  |  Issue : 2  |  Page : 108-115  

Ozone therapy in periodontics: A meta-analysis

Department of Periodontology, Rajarajeswari Dental College and Hospital, Bengaluru, Karnataka, India

Date of Submission27-Jan-2019
Date of Acceptance13-Dec-2019
Date of Web Publication07-Aug-2020

Correspondence Address:
Dr. Shivaprasad Bilichodmath
Department of Periodontology, Rajarajeswari Dental College and Hospital, #14 Ramohalli Cross, Kumbalgodu, Mysore Road, Bengaluru - 560 074, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ccd.ccd_79_19

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Gingival and periodontal diseases constitute a major distress in the field of dentistry. The greater part of the contributing and etiologic factors are diminished or treated with all forms of ozone. Ozone which is well known for its antimicrobial and tissue regenerative properties is indicated in all the stages of gingival and periodontal diseases. The aim of this study was to review literature on the effects of ozone as an adjunct to mechanical therapy in periodontitis patients. A search of literature was conducted to identify articles of ozone therapy in periodontitis published during the period from January 1, 2010, to July 30, 2018. PubMed, Medline, Embase, Cochrane, and Google Scholar search and hand searching of journals were conducted to identify relevant articles. The search strategy employed both dental subject headings and free-text terms. Out of a total of 123 studies that fit the initial inclusion criteria, 117 studies were further excluded. Only six studies were included in the meta-analyses. A high level of heterogeneity in the selected studies was found as demonstrated by Q-value of 10.241 and I2 value of 80.49%. However, the funnel plots showed symmetrical shape, with prevalence studies indicating absence of publication bias. Ozone therapy can be used effectively as an adjunct to scaling and root planing in the treatment of periodontitis.

Keywords: Aggressive periodontitis, chronic periodontitis, ozone therapy, scaling and root planing, subgingival debridement

How to cite this article:
Deepthi R, Bilichodmath S. Ozone therapy in periodontics: A meta-analysis. Contemp Clin Dent 2020;11:108-15

How to cite this URL:
Deepthi R, Bilichodmath S. Ozone therapy in periodontics: A meta-analysis. Contemp Clin Dent [serial online] 2020 [cited 2022 Oct 1];11:108-15. Available from:

   Introduction Top

Periodontitis is a complex disease which is marked by microbial and host response interaction. Microorganisms have also been found to be associated with the commencement of disease in the subgingival environment.[1]

Ecological plaque hypothesis suggests that a disease is not only hindered by suppressing the presumptive pathogens alone but also by impeding with the factors responsible for the transition of plaque microflora from the commensal to a pathogenic relationship with the host. The critical event in ecological plaque hypothesis is collapse of microbial hemostasis and shifting the ecological balance of plaque back toward one that is compatible with dental health. Thus, preventing strategies include altering subgingival environment which includes application of oxygenating agents. An alternative approach by the suppression of subgingival bacteria is to inhibit their growth by changing the subgingival environment (anaerobic) to aerobic.[1]

Ozone which is present in atmosphere is an uncolored gas form of oxygen. It is formed as a result of exposure to ultraviolet (UV) rays on a combination of three atoms of oxygen. It is also formed by the action of lightening discharges and has the capacity to absorb harmful UV rays.[1]

In 1785, Van Marum noticed that the air next to his electrostatic machine acquired a characteristic odor when electric sparks were passed. "Ozone" was named by Schonbein in the year 1840. It is derived from the Greek word "Ozein" meaning odorant. At very low temperature, ozone which is a pale blue gas will be condensed to a deep blue liquid. It is unstable and quickly gives up the nascent oxygen to form oxygen. Hence, the powerful oxidizer, ozone, is routinely used in human medicine to kill bacteria and fungi and to inactivate viruses. The nascent oxygen released by the spontaneous breakdown of ozone combines with water molecules to form hydroxyl group which is a more powerful oxidizer.[1]

Ozone is a powerful oxidant with distinct antimicrobial activity and has the capacity to act as a metabolic and host immune modulator. Ozone has been used for sterilization of cavities, root canals, and periodontal pockets, in the treatment of early carious lesions; to enhance epithelial wound healing, such as that caused by ulceration and herpetic lesions; as a rinse for avulsed teeth; and as a denture cleaner. The routes of administration of ozone for both gaseous and aqueous forms are topical and regional.[2] It has been documented that aqueous ozone possesses a distinctive biocompatibility to fibroblasts, cementoblasts, and epithelial cells, suggesting aptness of its use against oral infectious diseases such as periodontal disease, apical periodontitis, and peri-implantitis.[3]

Ozone in periodontitis treatment is used as an adjunctive to scaling and root planing (SRP) as compared to SRP alone.

Ozone has a role to play several actions in the human body such as an immune-stimulating, an analgesic, an antihypoxic, a detoxicating, an antimicrobial, a bioenergetic, and a biosynthetic agent.[4]

Ozone therapy as a contemporary noninvasive method of treatment is gaining popularity; it is a strong oxidizing agent with a high antimicrobial power against oral microorganisms, without developing resistance neither for gaseous, nor for aqueous ozone. It is used widely in varying treatment methods in the field of medicine, dentistry, veterinary, food industry, and water treatment.

Despite these potential beneficial effects, a meta-analysis of the effectiveness of ozone therapy in treating periodontitis has not been reported by studies conducted on humans. Furthermore, given the results of various studies and the absence of any previous meta-analyses, there is a great need to assess the literature systematically. The aim of the present meta-analysis is to evaluate the role of ozone therapy in treating periodontitis.

   Materials and Methods Top

Search criteria

  1. PubMed
  2. Medline
  3. Embase
  4. Sci-hub
  5. Cochrane
  6. Science Direct
  7. Google
  8. 2010–2018.

Focused question

How effective is ozone therapy as an adjunct in treating periodontitis?

  1. Population: Individuals with chronic periodontitis (CP) and aggressive periodontitis
  2. Intervention: Use of ozone therapy as an adjunct to mechanical SRP
  3. Comparison: Between ozone therapy group and placebo/no treatment group
  4. Outcome: Changes in pocket depth, clinical attachment level (CAL), bleeding on probing (BOP), Plaque Index (PI), and Gingival Index (GI)
  5. Selection criteria.

Inclusion criteria

  1. Publication of domestic and international peer-review literature
  2. Articles written in English
  3. Human randomized controlled trial
  4. More than 6 weeks of follow-up
  5. More than ten patients participating in the study
  6. Articles reporting data on change of pocket depth, clinical attachment loss, GI, PI, and BOP.

Parameters used

Probing pocket depth (PPD), clinical attachment loss, GI, PI, BOP, Gingival Bleeding Index, and Sulcular Bleeding Index.

Exclusion criteria

  1. In vitro studies
  2. Animal studies
  3. Retrospective cross-sectional studies.

Search strategy

A literature search was performed at Rajarajeswari Dental College and Hospital, Bengaluru, using electronic media in the following databases:, Cochrane database, EBSCO, Medline,, Sci-hub, and Embase between January 1, 2010, and July 30, 2018, using the following terms: Ozone therapy, aggressive periodontitis, chronic periodontitis, ozone nanobubble water, chlorhexidine, and subgingival debridement.

Two reviewers independently performed article search. They first examined the titles and abstracts of articles searched and selected papers for full-text screening. K value was introduced to examine agreement.

   Results Top

Our initial search resulted in the identification of 123 articles. Subsequently, 109 articles on the basis of title and abstract were excluded. Fourteen full-text article studies were considered to be potentially relevant for this review. Of these, we excluded five articles during full-text screening because they did not provide individual patient data or comparison between test and control groups.

In addition, we excluded two articles because they were review articles.

By the end of the search phase, we considered seven articles eligible; their data are the basis of review. Therefore, we cited the articles with shorter follow-up period. [Figure 1] is a flowchart of studies assessed and excluded at various stages of the review.
Figure 1: Flowchart of manuscript screened through the review process

Click here to view

Characteristics of the included studies

The seven included studies which fulfilled the eligibility criteria were clinical and either performed at universities or at oral health-care centers.

The number of participants ranged between 13 and 113 individuals and all were either CP or aggressive periodontitis patients. All individuals were adults and their age ranged between 20 and 72 years.

Most of the studies that fulfilled eligibility criteria were randomized controlled clinical trials. All the seven included studies for meta-analysis received SRP and subgingival application of ozone in different forms according to the test and control groups as divided by the examiner.

Outcome measures

The researchers observed changes in PPD, CAL, PI, GI, and BOP during the course of each study. Most of the investigators reported a statistically significant gain in CAL, PPD, BOP, PI, and GI.

In the meta-analysis of seven studies, comparing mechanical debridement alone and mechanical debridement + ozone-treated group, the results indicated a statistically significant reduction in PPD with the weighted mean difference of 0.03 (95% CI, −0.44, 0.50) and random effect model estimate of 0.0286. Reduction in PI, GI, and CAL was also seen with a weighted mean difference of −0.23 (95% CI, −0.64, 0.19), −0.45 (95% CI, −1.37, 0.46), and −0.26 (95% CI, −0.89, 0.37), respectively, with random effect model estimate of −0.226, −0.454, and −0.263, respectively.

   Discussion Top

In all of the seven studies analyzed in this review, the results showed that application of ozone in topical form reduced clinical parameters such as GI, PI, PPD, and CAL [Table 1].
Table 1: Characteristics of included studies

Click here to view

In 2014, Shoukheba and Ali evaluated the effect of subgingival application of ozonated olive oil gel as an adjunct to SRP in aggressive periodontitis patients. The control group which consisted of 15 patients received oral hygiene instructions, SRP of all teeth, in addition to the subgingival application of an ozonated olive oil gel (Oxaktiv ® gel). Clinical parameters such as PI, GI, BOP, and CAL were analyzed. The results of ozone irrigation showed improvement in all the clinical parameters in the ozone-treated group, which was maintained up to 6 months except for BOP up to 3 months.[2]

A prospective randomized clinical study was conducted by Gianluca Sacco in 2016 on 113 patients with periodontal disease. Local oxygen–ozone therapy was used in combination with traditional mechanical therapy versus the use of mechanical therapy alone in a group of patients with periodontal disease. After 6 months of root planing, the individuals who received oxygen–ozone therapy as an integral part of the periodontal treatment had significant stabilization of the clinical values. It was observed in particular that in both groups that the deepest pockets (PD > 6 mm) had the greatest decrease.[3]

Kshitish and Laxman developed a randomized, double-blind, crossover, split-mouth design. This split-mouth design was used in this study for subgingival irrigation of each half of the mouth with either ozone or chlorhexidine at different time intervals. The interpretation of clinical and microbial data is from baseline to the 7th day. The higher percentage of PI (12%), GI (29%), and Bleeding Index (26%) reduction was observed using ozone irrigation as compared to chlorhexidine.[4]

A study conducted by Yilmaz et al. evaluated the clinical and microbiological results of treatment with Er: yttrium aluminum garnet (YAG) laser and topical gaseous ozone application as adjuncts to initial periodontal therapy in CP patients. Thirty patients with CP were randomly divided into three parallel groups, each composed of ten individuals. Group 1: SRP + Er: YAG laser; Group 2: SRP + topical gaseous ozone; and Group 3: SRP alone. The clinical parameters were monitored at day 0 and day 90. Clinical parameters such as PI, SBI, PD, and relative attachment levels were measured at the end of the observation period; statistically significant improvements in clinical parameters were observed within each group.[5]

Al Habashneh et al.[6] determined the clinical and biological effects of the adjunctive use of ozone in nonsurgical periodontal treatment. Forty-one patients with CP were randomized to treatment with either subgingival SRP followed by irrigation with ozonated water for the test group or subgingival SRP followed by irrigation with distilled water irrigation for the control group. The parameters such as PI, GI, BOP, PPD, gingival recession, and clinical attachment loss were evaluated at baseline and at 3 months and statistically significant improvement was observed in the study parameters in both groups at baseline and 3 months, except for GI.

In a randomized study, Sae Hayakamo et al. evaluated the clinical and microbiological effects of NBW3 irrigation as an adjunct to subgingival debridement for periodontal treatment. Twenty-two patients were randomly assigned to one of the following two treatment groups: full-mouth mechanical debridement with tap water (WATER) or full-mouth mechanical debridement with NBW3 (NBW3). Full-mouth clinical measurements of PPD, CAL, and the percentage of BOP (%) were recorded at baseline and at 4 and 8 weeks after treatment. There were significant improvements in all the clinical parameters after 4 weeks in both groups. The reduction in the PPD and the clinical attachment gain after 4 and 8 weeks in the NBW3 group was significantly greater than that in the WATER group. BOP (%) was reduced by 15.69 and 8.98 at 4 weeks and 13.47 and 6.97 at 8 weeks in the NBW3 and WATER groups, respectively.[7]

Local ozone application can serve as a potential atraumatic, promising antimicrobial agent to treat periodontal disease nonsurgically, both for home care and professional practice. It may serve as a good tool during supportive periodontal therapy. Ozone may be considered as an alternative management strategy due to its powerful ability to inactivate microorganisms. Thus, subgingival ozone irrigation can be successfully used as an adjunct to periodontal treatment.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

Meta-Analysis (Control)

Plaque Index at the end of the study

Forest plot

Publication bias assessment

Funnel plot


Gingival Index at the end of the study

Forest Plot

Publication Bias Assessment

Funnel Plot


Probing pocket depth at the end of the study

Forest plot

Publication bias assessment

Funnel plot


Clinical attachment loss at the end of the study

Forest plot

Publication bias assessment

Funnel plot

   References Top

Vinutha RS, Lakshmanan R. Ozone and Its Role in Periodontal Therapy-A Review. IOSR-JDMS 2014;13:107-10. S 2014;13:107-10.  Back to cited text no. 1
Shoukheba MY, Ali S. The effects of subgingival application of ozonated olive oil gel in patient with localized aggressive periodontitis. A clinical and bacteriological study. Tanta Dent J 2014;11:63-73.  Back to cited text no. 2
Sacco G, Campus G. The treatment of periodontal disease using local oxygen-ozone. Ozone Ther 2017;1:45-52.  Back to cited text no. 3
Kshitish D, Laxman VK. The use of ozonated water and 0.2% chlorhexidine in the treatment of periodontitis patients: A clinical and microbiologic study. Indian J Dent Res 2010;21:341-8.  Back to cited text no. 4
[PUBMED]  [Full text]  
Yılmaz S, Algan S, Gursoy H, Noyan U, Kuru BE, Kadir T. Evaluation of the clinical and antimicrobial effects of the Er:YAG laser or topical gaseous ozone as adjuncts to initial periodontal therapy. Photomed Laser Surg 2013;31:293-8.  Back to cited text no. 5
Al Habashneh R, Alsalman W, Khader Y. Ozone as an adjunct to conventional nonsurgical therapy in chronic periodontitis: a randomized controlled clinical trial. J Periodontal Res 2015;50:37-43.  Back to cited text no. 6
Hayakumo S, Arakawa S, Mano Y, Izumi Y. Clinical and microbiological effects of ozone nano-bubble water irrigation as an adjunct to mechanical subgingival debridement in periodontitis patients in a randomized controlled trial. Clin Oral Investig 2013;17:379-88.  Back to cited text no. 7


  [Figure 1]

  [Table 1]

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