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 Table of Contents  
ORIGINAL ARTICLE
Year : 2017  |  Volume : 8  |  Issue : 1  |  Page : 102-105  

Interleukin 1β (+3954; −511) genotype polymorphism and its association with severe chronic generalized periodontitis in the Malaysian Population


Faculty of Dentistry, SEGi University, Selangor, Malaysia

Date of Web Publication24-Apr-2017

Correspondence Address:
Shelly Arora
SEGi University, No. 9, Jalan Teknologi, Taman Sains, Kota Damansara, Petaling Jaya, Selangor
Malaysia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ccd.ccd_1177_16

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   Abstract 


Introduction: Single-nucleotide polymorphisms (SNPs) in interleukin 1β (IL-1β) gene have been known to be associated with increased susceptibility to chronic periodontitis among various ethnic populations. SNPs are more commonly observed at loci + 3954 and − 511. The aim of this study was to evaluate the role of IL-1β gene polymorphism at loci +3954 and − 511, and its association with severe chronic generalized periodontitis among the ethnic Malay, Chinese, and Indians within the Malaysian population. Materials and Methods: Saliva samples from 120 subjects (60 cases and 60 controls) in the age group of 25–50 years were collected for isolation of genetic material using Norgen technique. Clinical attachment loss of ≥5 mm was considered as severe chronic generalized periodontitis. SNP's at loci +3954 and − 511 were identified and analyzed using Kompetitive Allele Specific Polymerase Chain Reaction Genotyping System (KASP). Differences in the allele/genotype frequencies were assessed by Chi-square test (P < 0.05). Results: On the comparison between cases and controls of IL-1β genotype polymorphism (+3954 and − 511), the difference in the genotype frequencies was statistically insignificant in all the three ethnicities. The genotype frequency in both groups in all three ethnicities of the Malaysian population was similar. Conclusion: IL-1β genotype polymorphism at +3954 and − 511 was found to be not associated with severe chronic generalized periodontitis among the three ethnicities in Malaysia. Studies with larger sample size should be done to confirm the findings of this study.

Keywords: Chronic generalized periodontitis, interleukin-1β genotype, Malaysian population, severe, single nucleotide polymorphism


How to cite this article:
Arora S, Ramachandra SS, Abdullah F, Gundavarapu KC. Interleukin 1β (+3954; −511) genotype polymorphism and its association with severe chronic generalized periodontitis in the Malaysian Population. Contemp Clin Dent 2017;8:102-5

How to cite this URL:
Arora S, Ramachandra SS, Abdullah F, Gundavarapu KC. Interleukin 1β (+3954; −511) genotype polymorphism and its association with severe chronic generalized periodontitis in the Malaysian Population. Contemp Clin Dent [serial online] 2017 [cited 2021 Dec 8];8:102-5. Available from: https://www.contempclindent.org/text.asp?2017/8/1/102/205034




   Introduction Top


Chronic periodontitis is an inflammatory disease affecting tissues of the periodontium resulting in clinical attachment loss (CAL) and bone loss and is one of the most common causes for tooth loss in developing nations.[1]

The prevalence of chronic periodontitis and severe chronic generalized periodontitis was found to be 48.5% and 18.2% respectively among Malaysian population.[2] Chronic periodontitis is multifactorial in nature with smoking, diabetes, osteoporosis, and hyper-responsive interleukin (IL) (genetic polymorphism) being some of the risk factors.[3] IL-1β is a key regulator of the host response and is a potent bone resorbing factor. It has been reported that single-nucleotide polymorphisms (SNPs) in IL-1β gene are associated with increased susceptibility to chronic periodontitis.[3] Various ethnic populations are known to have varying susceptibility to chronic periodontitis owing to SNPs in IL-1β gene.[4] Around 30% of the Caucasian population has IL-1β genotype polymorphism associated with increased susceptibility to chronic periodontitis.[4]

IL-1 gene complex comprised IL-1α, IL-1β, and IL-receptor antagonist are located on chromosome 2q13–21 and are polymorphic at several locations.[4] Studies have reported SNP's of IL-1β at +3954 and −

511 results in increased susceptibility to chronic periodontitis among various populations.[4] Thorough literature search did not reveal any published literature related to SNPs of IL-1β and its association with chronic periodontitis in the Malaysian population. Hence, the aim of this study was to evaluate the role of IL-1β gene polymorphism at loci +3954 and − 511, and its association with severe chronic generalized periodontitis among the various ethnicities within the Malaysian population.


   Materials and Methods Top


The study was conducted from May 2015 to March 2016 at Faculty of Dentistry, SEGi University in collaboration with Sengenics, Malaysia. The study was approved by Institutional Ethics Committee, SEGi University and informed consent was obtained from all the subjects participating in the study. The study comprised 120 subjects (60 cases and 60 controls) in the age group 25–50 years reporting to SEGi Oral Health Centre. Among 120 subjects, 40 each were from Chinese, Malay, and Malay-Indian ethnicities among the Malaysian population. The study subjects were selected from a homogeneous population with both parents being of the same ethnic origin. Controls were subjects with healthy periodontium without any systemic diseases. Cases included patients with CAL ≥5 mm according to American Academy of Periodontology, 1999.[5] All the participants had poor plaque scores (plaque index score of >2.0) and gingival index scores more than 2.0.[6] All examinations were done by a single calibrated examiner using a diagnostic mouth mirror and University of North Carolina-15 periodontal probe. Measurement of probing depth and clinical attachment level was recorded. The exclusion criteria were the presence of systemic diseases such as diabetes mellitus, obesity, acute infections, asthma, cardiovascular diseases, rheumatoid arthritis, and patients under antibiotic or nonsteroidal anti-inflammatory drugs in the past 6 months. Patients were requested to abstain from consuming food 2 h before providing saliva samples. Saliva samples (2–3 ml) were collected from all the patients in a sterile container and were sent to Sengenics lab, Malaysia for IL-1β (+3954; −511) genotype polymorphism analysis.

Norgen's Saliva DNA isolation kit (Norgen Biotek Corp., Thorold, ON, Canada as per manufacturer advised protocols) was used for the extraction of whole genomic DNA.[7] The extracted DNA was quantified using ThermoFisher Scientific Qubit ® 3.0 Fluorometer (Thermo Fisher Scientific, Massachusetts, U.S.A). SNP's analysis was completed utilizing Kompetitive Allele Specific polymerase chain reaction (PCR) genotyping system (KASP ).[7] 17.5 μg DNA of every example was aliquoted into every well in 384-well plate separately twice, and the plate was dried in an oven at 60°C for 60 min. After the DNA in the 384-well plate was totally dry, dispense 3 μL of diluted KASP Master mix containing 250 μL concentrated KASP Master mix (LGC Genomic Limited, Middlesex, United Kingdom), 250 μL laboratory grade water and 7 μL of IL-1β (−511) assay mix (rs16944) using Meridian (LGC Genomic Limited, Middlesex, United Kingdom) to the first set of patient's sample and diluted KASP Master mix with IL-1β (+3954) assay mix (rs1143634) to second set of patient's sample. The plate was secured with clear seal and amplified using water bath PCR thermal cycler, Hydrocycler 16 (LGC Genomic Limited, Middlesex, United Kingdom). The PCR cycling condition used by Hydrocycler 16 was 61°C–55°C [Table 1]. PHERAstar (BMG Labtech, Offenburg, Germany) was used to scan the plate. The SNP genotype was analyzed through Kraken software and validated by laboratory technologist.
Table 1: Polymerase chain reaction amplification thermal cycling condition used by Hydrocycler 16

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Statistical analysis done

Data obtained was tabulated and statistically analyzed using MedCalc version 12 (Acacialaan 22, 8400 Ostend, Belgium). Paired t-test was applied to compare the age distribution among the three ethnicities of the Malaysian population. Chi-square test of proportions was carried out to compare the difference between cases and controls of genotype and allele frequencies distribution. The program package Arlequin 3.0 (Arlequin, Suisse, Institute of Ecology and Evolution, University of Bern) was used to calculate P value using Hardy–Weinberg equation (HWE).[8] Differences were considered statistically significant when P< 0.05.


   Results Top


This study recruited 120 subjects consisting of 60 healthy volunteers and sixty patients with severe chronic generalized periodontitis (40 Chinese, 40 Malaysians, and 40 Malay-Indians). There were 50 males and 70 females in the age range of 24–50 years (mean age = 35.3 years). There was no significant difference in age distribution of patients between cases and controls under each ethnicity [Table 2]. [Table 3] shows the gender distribution in each ethnicity.
Table 2: Mean age difference between cases and controls

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Table 3: Gender comparison between cases and controls

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In ethnic Malays, IL-1β genotype at +3954 among cases was 17 CC and 3 computed tomography (CT), and in controls, there were 17 CC and 3 CT. Whereas IL-1β genotype at −511 in Malay cases was 6 CC, 8 CT, and 6 TT and among controls, there were 7 CC, 6 CT, and 7 TT [Table 4].
Table 4: Association analysis of genotypes of single nucleotide polymorphism of interleukin 1β gene with chronic periodontitis in ethnic Malays, Chinese and Indians

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Among ethnic Chinese, IL-1β genotype at +3954 in cases was found to be predominantly composed of CC genotype only whereas in controls there were 18 CC and 2 CT genotypes.

At the locus −511 genotype among cases were distributed as 6 CC, 9 CT, and 5 TT whereas, in controls, it was found to be 8 CC, 8 CT, and 4 TT [Table 4].

Among ethnic Malay-Indians, the prevalence of IL-1β genotype at + 3954 in cases was found to be predominantly composed of CC genotype (14) followed by CT (4) and TT (2). Whereas in controls IL-1β genotype at +3954 was distributed as 15 CC and 5 CT. IL-1β genotype at −511 among cases was found to be 4 CC, 8 CT, and 8 CC, whereas in controls it was found to be 4 CC, 6 CT, and 10 TT [Table 4].

The genotype frequency of IL-1β (+3954) and IL-1β (−511) was compared between cases and controls in Malays, Chinese, and Malay-Indians residing in Malaysia. The genotype frequency of both IL-1β (+3954) and (−511) in all the three ethnicities did not deviate from HWE. On the comparison between cases and controls among IL-1β (+3954) as well as IL-1β (−511), the difference in genotype of distribution was statistically insignificant in all the three ethnicities. The pure allelic contribution of TT for IL-1β (+3954) polymorphism was absent in both cases as well as controls in ethnic Malays and Chinese. Thus, the genotype frequency in both groups in all three populations falls within the comparable limits.


   Discussion Top


Gene polymorphism resulting in increased production of cytokines has been considered as a contributing factor in the pathogenesis of chronic generalized periodontitis.[9] In this study, DNA was isolated from saliva samples of 120 subjects. Noninvasiveness, collection of samples by nontrained individuals and without any specific equipment are the various advantages of using saliva as a source for DNA isolation.[7] In this study, SNP was identified using KASP genotyping. SNP genotyping using PCR-RFLP and KASP have shown similar results.[10]

Masamatti et al. studied 90 subjects (30 chronic periodontitis, 30 aggressive periodontitis, and 30 healthy controls) for IL-1β at + 3954 among the South Indian population.[11] The results showed a positive association between IL-1β genetic polymorphism and chronic periodontitis, whereas no positive association was observed between IL-1β genetic polymorphism and aggressive periodontitis.[12] Archana et al. studied sixty patients (15 healthy and 15 each for mild, moderate, and severe chronic periodontitis) for the association between IL-1β polymorphism and chronic periodontitis.[12] The results showed a significant association between severe periodontitis and IL-1β genetic polymorphism.[12] In our study, we have evaluated only severe chronic generalized periodontitis patients. Wu et al. studied the association of IL-1 gene variations with moderate to severe chronic periodontitis in four ethnic groups (Caucasians, African Americans, Hispanics, and Asians). The patients were recruited in USA, Chile, and China. The study revealed that IL-1β (+3954) gene variations resulted in the significant effect on the severity of chronic periodontitis in all the four ethnic groups studied.[4]

Armitage et al., in 2000, studied 300 subjects of Chinese origin for IL-1β +3954 gene polymorphism and its association to the severity of chronic periodontitis. The study concluded that the prevalence of IL-1B polymorphisms was lower in Chinese subjects. However, these patients were from Chinese heritage and not from China. This study questioned the usefulness of IL-1β polymorphism as a method for determining the susceptibility of Chinese patients to chronic periodontitis.[13] Anusaksathien et al. studied 123 Thai subjects categorized into chronic periodontitis, aggressive periodontitis, and healthy groups. The IL-1β (+3954) genotype was studied using polymerase chain reaction.[14] The study concluded that genetic polymorphism of IL-1β gene at +3954 locus was not useful in predicting the severity of periodontal disease in the Thai ethnic group.[14] The results of this study conducted in the South East Asian region is supporting the findings found in our study.

Yücel et al. studied SNP's in IL-1β gene at the locus +3954 among 44 chronic periodontitis patients and 47 healthy controls of Turkish origin.[15] The genotype distribution and allele frequencies were not different after stratification of subjects according to CAL. Among chronic periodontitis patients, sites with increased bleeding on probing showed an increased frequency of allele 2. The study concluded that SNP's at +3954 in IL-1β gene could potentially play a significant but not major role in the clinical outcome.[15] Our study considered patients with severe periodontitis only.


   Conclusion and Shortcomings of the Study Top


Our study evaluated the IL-1β genotype polymorphism at loci +3954 and −511 in three ethnic groups residing in Malaysia. The study showed no association between severe chronic generalized periodontitis and IL-1β genotype polymorphism at the above-mentioned loci. The population studied in each group was just 40 subjects. The study should be extended to include a larger sample size for substantiating the results obtained from this study.


   Acknowledgment Top


Mr. Johan Poole-Johnson, Commercial Director, Sengenics, Malaysia, Dr. Arif Anwar, Sengenics, Malaysia, Ms. Farah Aziz, Laboratory Operations Manager, Sengenics, Malaysia. Research and Innovation Management Center, SEGi University, Selangor, Malaysia.

Financial support and sponsorship

Research and Innovation Management Center, SEGi University, Selangor, Malaysia.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Khan S, Saub R, Vaithilingam RD, Safii SH, Vethakkan SR, Baharuddin NA. Prevalence of chronic periodontitis in an obese population: A preliminary study. BMC Oral Health 2015;15:114.  Back to cited text no. 1
    
2.
NOHSA. National Oral Health Survey in Adults 2010. Putrajaya: Oral Health Division Ministry of Health; 2013.  Back to cited text no. 2
    
3.
Loos BG, Papantonopoulos G, Jepsen S, Laine ML. What is the contribution of genetics to periodontal risk? Dent Clin North Am 2015;59:761-80.  Back to cited text no. 3
    
4.
Wu X, Offenbacher S, López NJ, Chen D, Wang HY, Rogus J, et al. Association of interleukin-1 gene variations with moderate to severe chronic periodontitis in multiple ethnicities. J Periodontal Res 2015;50:52-61.  Back to cited text no. 4
    
5.
Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999;4:1-6.  Back to cited text no. 5
    
6.
Löe H. The gingival index, the plaque index and the retention index systems. J Periodontol 1967;38 Part-II:610-6.  Back to cited text no. 6
    
7.
KASP Genotyping Chemistry User Guide and Manual. Available from: https://www.lgcgroup.com/LGCGroup/media/PDFs/Products/Genotyping/KASP-genotyping-chemistry-User-guide.pdf. [Last accessed on 2016 Oct 8].  Back to cited text no. 7
    
8.
Excoffier L, Laval G, Schneider S. Arlequin (version 3.0): An integrated software package for population genetics data analysis. Evol Bioinform Online 2007;1:47-50.  Back to cited text no. 8
    
9.
Kadkhodazadeh M, Ebadian AR, Amid R, Youssefi N, Mehdizadeh AR. Interleukin 17 receptor gene polymorphism in periimplantitis and chronic periodontitis. Acta Med Iran 2013;51:353-8.  Back to cited text no. 9
    
10.
Hubácek JA, Pikhart H, Peasey A, Kubínová R, Bobák M. Nobody is perfect: Comparison of the accuracy of PCR-RFLP and KASP™ method for genotyping. ADH1B and FTO polymorphisms as examples. Folia Biol (Praha) 2015;61:156-60.  Back to cited text no. 10
    
11.
Masamatti SS, Kumar A, Baron TK, Mehta DS, Bhat K. Evaluation of interleukin -1B (+3954) gene polymorphism in patients with chronic and aggressive periodontitis: A genetic association study. Contemp Clin Dent 2012;3:144-9.  Back to cited text no. 11
[PUBMED]  [Full text]  
12.
Archana PM, Salman AA, Kumar TS, Saraswathi PK, Panishankar KH, Kumarasamy P. Association between interleukin-1 gene polymorphism and severity of chronic periodontitis in a South Indian population group. J Indian Soc Periodontol 2012;16:174-8.  Back to cited text no. 12
[PUBMED]  [Full text]  
13.
Armitage GC, Wu Y, Wang HY, Sorrell J, di Giovine FS, Duff GW. Low prevalence of a periodontitis-associated interleukin-1 composite genotype in individuals of Chinese heritage. J Periodontol 2000;71:164-71.  Back to cited text no. 13
    
14.
Anusaksathien O, Sukboon A, Sitthiphong P, Teanpaisan R. Distribution of interleukin-1beta(+3954) and IL-1alpha(-889) genetic variations in a Thai population group. J Periodontol 2003;74:1796-802.  Back to cited text no. 14
    
15.
Yücel OO, Berker E, Mescil L, Eratalay K, Tepe E, Tezcan I. Association of interleukin-1 beta (+3954) gene polymorphism and gingival crevicular fluid levels in patients with aggressive and chronic periodontitis. Genet Couns 2013;24:21-35.  Back to cited text no. 15
    



 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4]


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