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  Indian J Med Microbiol
 

Figure 1: (a) Forty-five extracted human premolars; (b) teeth after restoration; (c) samples in thermostat; and (d) apical seal with acrylic and varnish application

Figure 1: (a) Forty-five extracted human premolars; (b) teeth after restoration; (c) samples in thermostat; and (d) apical seal with acrylic and varnish application