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  Indian J Med Microbiol
 

Figure 2: (a) Nail varnish applied (b) Samples kept in methylene blue solution for 24 H (c) teeth kept in 6 ml of 65% nitric acid for 3 days (d) Centrifuged at 5000 Rpm for 15 min (e) coagulum formed after centrifugation (f) automatic spectrophotometer reading

Figure 2: (a) Nail varnish applied (b) Samples kept in methylene blue solution for 24 H (c) teeth kept in 6 ml of 65% nitric acid for 3 days (d) Centrifuged at 5000 Rpm for 15 min (e) coagulum formed after centrifugation (f) automatic spectrophotometer reading