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Year : 2018  |  Volume : 9  |  Issue : 2  |  Page : 298-303

The anti-inflammatory effects of glycerol-supplemented probiotic lactobacillus reuteri on infected epithelial cells In vitro

1 Department of Microbiology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia
2 Microbiology Center of Research and Education, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia
3 Departement of Oral Biology, Faculty of Dentistry, University of Indonesia, Jakarta, Indonesia

Correspondence Address:
Dr. Armelia Sari Widyarman
Department of Microbiology, Faculty of Dentistry, Trisakti University, Jln. Kyai Tapa 260, Grogol Jakarta Barat, Jakarta 11440
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ccd.ccd_53_18

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Background: One of the most interesting effects of probiotics is their ability to modulate the immune system through the induction of cytokines and to enhance the host immune response. Aims: The purpose of this study was to evaluate the anti-inflammatory effect of glycerol-supplemented Lactobacillus reuteri on the transcription level of interleukin (IL)-8 and human-beta-defensin (hBD)-2 expressed by epithelial cells after exposure to bacteria. Materials and Methods: The confluent-cultured HaCat cell line (105 cells/mL) was exposed to Streptococcus mutans ATCC-25175 and Porphyromonas gingivalis ATCC-33277 (107 colony-forming units [CFU]/mL) for 24 h and challenged with probiotic L. reuteri ATCC-55730 (107 CFU/mL) supplemented with glycerol. Subsequently, the transcription levels of IL-8 and hBD-2 in HaCat cells were analyzed using reverse-transcription polymerase chain reaction (RT-PCR). In addition, cell viability was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. All the obtained data were statistically analyzed using the one-way analysis of variance test, with P < 0.05 set as the level of significance. Results: The MTT assays confirmed no cytotoxic effects of glycerol-supplemented L. reuteri on HaCat cells (viability >90%). mRNA expression of IL-8 and hBD-2 increased after exposure to both bacteria. The presence of glycerol-supplemented L. reuteri significantly reduced the expression of IL-8 and hBD-2 on HaCat cells (P < 0.05). Conclusion: Glycerol-supplemented L. reuteri reduced the expression of IL-8 and hBD-2, and the results may be proof of principle for a probiotic approach to combating inflammation. However, further studies are needed to validate this probiotic effect.

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