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 Table of Contents  
ORIGINAL ARTICLE
Year : 2017  |  Volume : 8  |  Issue : 1  |  Page : 33-37  

In vitro comparative evaluation of cleaning efficacy and volumetric filling in primary molars: Cone beam computed tomography evaluation


Department of Paedodontics and Preventive Dentistry, K. M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India

Date of Web Publication24-Apr-2017

Correspondence Address:
Anshula Neeraj Deshpande
Department of Paedodontics and Preventive Dentistry, K. M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-237X.205064

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   Abstract 


Introduction: Pulpectomy of primary teeth is mostly carried out with hand files and broaches which is tricky and time consuming procedure. The development of new design features like varying tapers, non-cutting safety tips and varying length of cutting blades have resulted in new generation of rotary instruments. Aim: To compare and evaluate cleaning efficacy, canal preparation and volumetric filling using conventional files and rotary V Taper files through cone beam computed tomography. Materials and Method: Thirty extracted primary molars were selected. The teeth were randomly divided into three groups each containing 10 teeth i.e. 30 canals in each group. Group A was instrumented with K files; Group B rotary V Taper files and Group C was Hybrid group. Sodium hypochlorite (1%) was used for irrigation. Root canal filling was done with Zinc Oxide Eugenol cement in all groups. The volumetric analysis i.e. Percentage of Volume (POV) of the root canal filling in primary molars was done through CBCT Software. Result: In present study, p- value was found to be significant (<0.05). Almost 100% of canals of hybrid group were fully filled and 63.3% of canals of hand filing group were partially filled. The filling was found to be dense and no. of voids was least in hybrid group. Conclusion: Clinical time required in primary molar endodontics, especially with unpredictability and difficulty of canal morphology, is inevitable. The study confirms superior ability of rotary-file systems to shape severely curved canals with less time and significant decrease in procedural errors like partial filling, voids and inappropriate canal preparation.

Keywords: Primary teeth, pulpectomy, rotary, V-taper, zinc oxide eugenol cement


How to cite this article:
Deshpande AN, Joshi NH, Naik KS. In vitro comparative evaluation of cleaning efficacy and volumetric filling in primary molars: Cone beam computed tomography evaluation. Contemp Clin Dent 2017;8:33-7

How to cite this URL:
Deshpande AN, Joshi NH, Naik KS. In vitro comparative evaluation of cleaning efficacy and volumetric filling in primary molars: Cone beam computed tomography evaluation. Contemp Clin Dent [serial online] 2017 [cited 2019 Jul 17];8:33-7. Available from: http://www.contempclindent.org/text.asp?2017/8/1/33/205064




   Introduction Top


One of the most important concerns in pediatric dentistry is the loss of necrotic primary molars, leading to space loss. Although the morphology of root canals in primary teeth renders endodontic treatment difficult,[1],[2],[3] pulpectomy of primary teeth with severe pulpal involvement should be considered as a treatment of choice. The success of pulpectomy depends on elimination of irritants by means of cleaning and shaping the root canal.[4] The primary objectives of cleaning and shaping the root canal system are removing soft and hard tissue containing bacteria, providing a path for irrigants to the apical third, supplying space for medicaments and subsequent obturation, retaining the integrity of radicular structure.[2]

The nature of canal dimensions, shape, and curves as well as the physical properties of instruments prevents the possibility of a uniform, tapered, flowing preparation. Canal shaping is relatively easy in straight roots but has always been challenging, demanding a high skill, when performed in curved roots.[5] Many complications such as ledging, apical perforation, and mid-root strip perforation may affect the long-term success of treatment as it fails to eliminate infection of the root canal system and hence making the obturation more difficult. Various instrumentation techniques and instruments have been introduced in an attempt to reduce these problems aiming to provide the optimum-shaped preparation. The development of new design features such as varying tapers, noncutting safety tips, and varying length of cutting blades has resulted in a new generation of instruments.[6]

According to the manufacturers, the V-Taper rotary system is a series of three variable taper NiTi rotary files. With this system, most molar and premolar root canal preparation can be completed using 2–3 files and anterior root canals using 1–2 files. This performance-enhanced system is easier, safer, more efficient, and less expensive than any other NiTi rotary file system.[6]

With the introduction of new system in field of rotary endodontic, the study with an aim to compare and evaluate the cleaning efficacy, canal preparation, and volumetric filling using conventional files and rotary V-Taper files through cone beam computed tomography (CBCT) was carried out.


   Methodology Top


For the present study, ethical clearance was obtained from the Institutional Ethical Committee of Sumandeep Vidyapeeth (Approval No. SVIEC/ON/Dent/RP/15036). The minimum requirement of sample was 28 observations per group with following assumptions.[7] Mean difference of percentage of voids (PV) between two groups: 0.6; combined standard of deviation: 0.8; effect size: 0.75; confidence interval: 95%; power: 80%; test: two-tailed. Hence, according to the above-mentioned assumptions, 30 extracted primary molars with minimum 3 canals in each tooth (90 canals) were distributed equally in all the three groups.

Thirty extracted primary molars with at least two-thirds of intact root and 7–12 mm length were included in the study [Figure 1]. Before starting, the teeth were cleaned with soap and washed in running water. Hand scalers were used to remove calculus if present on the root surface and stored in 0.5% sodium hypochlorite for 1 week. Coronal access was made with round diamond burs. After irrigation of the root canal with normal saline, a K-file with a compatible diameter was introduced into the root canal and the canal length was determined at 1 mm from the apex or root bevel. The teeth (30 teeth, 90 canals) were randomly divided into three groups. Group A (30 canals) was manually prepared with K-files up to a file size three times larger. Group B (30 canals) was instrumented with rotary V-Taper files up to a file size three times larger. Group C (30 canals) was instrumented first with K-files, followed by rotary V-Taper file in the same manner. Patency and working length of each canal were determined by passing the size #10 K-file. Sodium hypochlorite (1%) was used for irrigation through a 31-gauge needle after use of each instrument. Each root canal was irrigated with a total of 5 mL sodium hypochlorite. Canal recapitulation was performed after the use of each file. Files were regularly wiped using wet gauze to remove tissue debris.
Figure 1: Consolidated flowchart of methodology

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After canal preparation, root canal filling was done with zinc oxide eugenol cement using lentulospirals in all groups. The postendodontic investigations were done with CBCT. The volumetric analysis, i.e., percentage of volume (POV) of the root canal filling in primary molars, was done through CBCT software [Figure 2] and [Figure 3]. The collected data were entered in the master chart prepared on a computer using Microsoft Excel 2007, and the data were analyzed using SPSS software version 21 for descriptive test (standard deviation, coefficient of variation, mean) and independent test. Rotary files were discarded after five times of use. Infection control protocols were followed during the study, and the extracted primary molars were discarded after the completion of study following the infection control protocols.
Figure 2: Slice of cone beam computed tomography Image of arch with mounted teeth at cementoenamel junction level and at long axis

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Figure 3: Slice of cone beam computed tomography Image of arch with mounted teeth at furcation level

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   Results Top


The present in vitro study was done to evaluate the cleaning efficacy and volumetric filling in primary molars using CBCT.

Intragroup and intergroup comparative evaluations were done between Group A (hand filing), Group B (rotary filing), and Group C (hybrid filing) [Table 1]. P value was found to be highly significant (<0.001) in most cases, except coronal preoperative and postoperative observation between hand and rotary filing group, and also in the middle preoperative observation between hand and rotary filing group.
Table 1: Group comparison in relation to canal enlargement

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The quality of zinc oxide eugenol filling which is done in all the three groups, i.e., Group A (hand filing), Group B (rotary filing), and Group C (hybrid filing), is shown in [Table 2]. P value was highly significant (<0.001). Furthermore, we can appreciate that the Group C (hybrid filing) showed fully filled canal (n = 30) and Group A (hand filing) showed partially filled canal (n = 19) [Figure 4] and [Figure 5].
Table 2: Assessment of quality of filling

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Figure 4: Preoperative cone beam computed tomography image of 3 groups in vertical plane

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Figure 5: Postoperative cone beam computed tomography image of Volumetric filling of 3 groups in vertical plane

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Volumetric filling analysis was done by considering three variables, i.e., density of filling in coronal, middle, and apical area, also by considering the number of voids present in the canal filling [Table 3]. The comparison shows that density of filling in coronal area was found to be 5262 in hand filing group and 4202 in hybrid group. Further, least number of voids was present in hybrid group (0.13) [Figure 6].
Table 3: Volumetric filling analysis

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Figure 6: Cone beam computed tomography image of voids in root canal filling of 3 groups in horizontal plane

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We can appreciate that hand filing group had highest number of voids and least voids were found in Group C (hybrid group) [Figure 7].
Figure 7: Percentage of voids in root canal filling on cone beam computed tomography

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   Discussion Top


There is overwhelming evidence that the reduction of intracanal microorganisms is the major objective of endodontic therapy. The essential goals that the clinician must accomplish with root canal treatment are complete disinfection of the canal space, elimination of the progression of the periradicular tissue inflammation, and thereby creation of favorable conditions for periradicular healing. This can be achieved using a proper chemomechanical preparation of the root canals, which is fundamental for successful endodontic treatment.[8],[9]

However, conventional hand instruments often failed in achieving these objectives. Most canals are curved, whereas endodontic instruments are fabricated from straight metal blanks. This results in uneven force distribution in certain contact areas and tendency of the instrument to straighten itself inside the canal. Subsequently, apical canal areas have a tendency to be over prepared toward the outer curve or the convexity of the canal, whereas more coronal areas are transported toward the concavity.[5],[10] Similar results were observed in our study as there was difference in canal preparation among the three groups where more dentin was removed in conventional group > hybrid group > rotary group [Table 3].

Rotary biomechanical preparation of deciduous teeth was first described by Barr et al.[11] There are no clear guidelines for the instrumentation of primary teeth root canals with rotary files. Kuo et al.[12] found that with the modified protocol, ProTaper Ni-Ti rotary files can be safely and efficiently used for root canal preparation in primary molars. Considering the advantages of V-Taper Ni-Ti files, we have used them in our present study.

Advantages of V-Taper 2 rotary files are (1) deep apical shaping with conservative coronal shaping (2) deep apical shape creates better access for irrigation and cleaning, and three-dimensional obturation, (3) variable taper design creates conservative coronal shape, hence preserves dentin, (4) 1–2 files per case, lowest cost for shaping per root canal procedure, and (5) strongest tested file system on the market.

There are few studies that compare the cleaning ability of manual and rotary instrumentation in primary teeth. The current study compared the cleaning efficacy of manual instrument (K-files), rotary system (V-Taper) in the preparation of primary molar root canals.

To check the cleaning efficacy and volumetric filling, comparison was done between V-taper rotary files and Hand K-files. In addition, it is evident that the hybrid technique, i.e., the use of both hand and rotary files is beneficial; this technique was also taken into consideration. Hence, the three groups decided for the study were Group A (hand filing), Group B (rotary filing), and Group C (filing with hand and rotary files.).

Our study demonstrated a significant difference in the cleaning ability of rotary and hybrid filing group in coronal third (P < 0.0001), middle third (P < 0.0001), and apical third (P < 0.001) of the primary molar root canal as compared to manual instrumentation. Huang et al.[13] and Kadhom and Hashimi [14] showed the similar degree of cleaning efficacy in coronal third and middle third of the root canal.

Although the canal was cleaned thoroughly, dentin loss was also seen in the experimental groups and was the least seen in Group A, i.e., hand filing group. Canal enlargement was superior when rotary files were used. Similar results were also obtained by Nazari Moghaddam in 2009.[6] Due to varying taper of the V-Taper rotary files, canal shaping was uniformly conical and the chances of ledge formation were also reduced. P value (<0.05) was found to be highly significant when intra- and inter-group comparison was done.

Obturation of the root canal space will prevent the re-entry and growth of microorganisms and traps the traces of leftover pathogens inside the root canal system by a fluid tight seal. It likewise prevents the entrance of bacteria and their by-products from oral cavity and periapical area into obturated root canals, failing which it risks the endodontic treatment achievement.[15] Hence, assessing the quality of root canal filling as the final phase of root canal treatment is essential. For sealing root canal system, the filling must adapt to all parts of the canal. Inadequate obturation of the canal system may result in failure of the endodontic treatment. Root canals which were prepared by rotary files produced a conical pathway allowing effortless entrance of obturating paste and therefore less overfilling or underfilling and diminished odds of voids.

Density of filling was found to be better in the hybrid and rotary filing group in comparison with hand filing group. PV was found to be least in hybrid group (3% voids), followed by rotary group (10% voids) and highest in hand filing group (14%). Varieties of rotary systems are accessible commercially, yet one needs to choose prudently considering each canal morphology as one of a kind keeping in mind the end goal to avoid untoward iatrogenic mistakes. According to Peters, an important mechanical objective is to leave as much radicular dentin as possible so as not to weaken the root structure, accordingly preventing vertical fractures. Although no definitive minimal radicular thickness has been established, 0.2 mm is considered critical.[16],[17],[18] Further research is needed to confirm and elaborate on its canal transportation, uninstrumented surface area, and preservation of dentin thickness which influences the prognostic stability of the teeth.


   Conclusion Top


The young patient and parents will appreciate every minute saved with V-Taper rotary file. The greatest canal enlargement was found in hybrid group > rotary group > hand filing group. The greatest POV was obtained in hand filing group > rotary filing group > hybrid group. The results of the present investigation confirm the superior ability of using hybrid method (rotary files and hand files) for canal preparation and volumetric filling in primary molars.

Acknowledgment

We would like to acknowledge Nidaan Dental Care Private Limited (Dent-CAT).

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
McDonald RE, Avery DR. Dentistry for the Child and Adolescent. 7th ed. St. Louis: Mosby; 2000. p. 401.  Back to cited text no. 1
    
2.
Cohen S, Hargreaves KM. Pathways of the Pulp. 9th ed. St. Louis: Mosby; 2006. p. 301-11, 842.  Back to cited text no. 2
    
3.
Siqueira JF Jr., Araújo MC, Garcia PF, Fraga RC, Dantas CJ. Histological evaluation of the effectiveness of five instrumentation techniques for cleaning the apical third of root canals. J Endod 1997;23:499-502.  Back to cited text no. 3
    
4.
Yang SF, Rivera EM, Walton RE, Baumgardner KR. Canal debridement: Effectiveness of sodium hypochlorite and calcium hydroxide as medicaments. J Endod 1996;22:521-5.  Back to cited text no. 4
    
5.
Mohammadzade Akhlaghi N, Khalilak Z, Baradaran Mohajeri L, Sheikholeslami M, Saedi S. Comparison of canal preparation pattern of K3 and ProTaper rotary files in curved resin blocks. Iran Endod J 2008;3:11-6.  Back to cited text no. 5
    
6.
Nazari Moghaddam K, Mehran M, Farajian Zadeh H. Root canal cleaning efficacy of rotary and hand files instrumentation in primary molars. Iran Endod J 2009;4:53-7.  Back to cited text no. 6
    
7.
Naseri M, Kangarlou A, Khavid A, Goodini M. Evaluation of the quality of four root canal obturation techniques using micro-computed tomography. Iran Endod J 2013;8:89-93.  Back to cited text no. 7
    
8.
Hegde J, Bashetty K, Kumar KK, Chikkamallaiah C. Comparative evaluation of the sealing ability of different obturation systems used over apically separated rotary nickel-titanium files: An in vitro study. J Conserv Dent 2013;16:408-12.  Back to cited text no. 8
[PUBMED]  [Full text]  
9.
Ounsi HF, Franciosi G, Paragliola R, Al-Hezaimi K, Salameh Z, Tay FR, et al. Comparison of two techniques for assessing the shaping efficacy of repeatedly used nickel-titanium rotary instruments. J Endod 2011;37:847-50.  Back to cited text no. 9
    
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Danforth RA, Dus I, Mah J. 3-D volume imaging for dentistry: A new dimension. J Calif Dent Assoc 2003;31:817-23.  Back to cited text no. 10
    
11.
Barr ES, Kleier DJ, Barr NV. Use of nickel-titanium rotary files for root canal preparation in primary teeth. Pediatr Dent 2000;22:77-8.  Back to cited text no. 11
    
12.
Kuo CI, Wang YL, Chang HH, Huang GF. Application of Ni-Ti rotary files for pulpectomy in primary molars. J Dent Sci 2006;1:10-5.  Back to cited text no. 12
    
13.
Huang QL, Zhang XQ, Deng GZ, Huang SG. SEM evaluation of canal cleanliness following use of ProTaper hand-operated rotary instruments and stainless steel K-files. Chin J Dent Res 2009;12:45-9.  Back to cited text no. 13
    
14.
Kadhom TH, Hashimi WN. A study to compare the efficiency of different instrumentation systems for cleaning oval-shaped root canals (An in vitro study). J Baghdad Coll Dent 2013;25:49-55.  Back to cited text no. 14
    
15.
Raghavendra JM, Latha P, Gowda B, Manvikar V, Vijayalaxmi DB, Ponangi KC. Smear layer and debris removal using manual Ni Ti files compared with rotary Protaper Ni Ti files - An in vitro SEM study. J Int Oral Health 2014;6:89-94.  Back to cited text no. 15
    
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Stern S, Patel S, Foschi F, Sherriff M, Mannocci F. Changes in centering and shaping ability using three nickel titanium instrumentation techniques analysed by micro computed tomography (μCT). Int Endod J 2012;45:514-23.  Back to cited text no. 16
    
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Peters OA, Peters CI. Cleaning and shaping of root canal system. Cohen's Pathways of the Pulp. 10th ed. UK: Elsevier; 2011. p. 290-357.  Back to cited text no. 17
    
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Peters OA, Peters CI, Schönenberger K, Barbakow F. ProTaper rotary root canal preparation: Assessment of torque and force in relation to canal anatomy. Int Endod J 2003;36:93-9.  Back to cited text no. 18
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7]
 
 
    Tables

  [Table 1], [Table 2], [Table 3]



 

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