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ORIGINAL ARTICLE
Year : 2015  |  Volume : 6  |  Issue : 6  |  Page : 227-234

C-deletion in exon 4 codon 63 of p53 gene as a molecular marker for oral squamous cell carcinoma: A preliminary study


1 Department of Oral Pathology, Index Institute of Dental Sciences, Indore, Madhya Pradesh, India
2 Department of Oral Pathology, S.R.M. Dental College and Hospital, Ramapuram, Chennai, Tamil Nadu, India
3 Department of Oral Pathology, Sree Balaji Dental College and Hospital, Bharath University, Chennai, Tamil Nadu, India
4 Department of Pharmacology, Index Medical College Hospital and Research Centre, Indore, Madhya Pradesh, India

Correspondence Address:
Sukanta Sen
Department of Pharmacology, Index Medical College Hospital and Research Centre, Indore, Madhya Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-237X.166840

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Background: Exfoliated oral cancer cells in saliva samples from patients with oral squamous cell carcinoma (OSCC) can be used to determine the incidence and type of mutations of the p53 tumor suppressor gene. The purpose of this study was to identify C-deletion mutation in exon 4 codon 63 of p53 gene in the saliva of OSCC patients by polymerase chain reaction (PCR). Materials and Methods: Saliva samples of 20 newly histopathologically diagnosed OSCC patients and 5 healthy volunteers were subjected to isolation of the total genomic DNA and PCR amplification for C-deletion on exon 4 of p53 gene. The resulting products were resolved by agarose gel electrophoresis, viewed and photographed on ultraviolet-transilluminator. Results: The relationship between the frequencies of genetic alterations was assessed by Chi-square test. Differences with values of P < 0.05 were statistically significant. Conclusion: The study concluded a 100% presence of C-deletion mutation in exon 4 codon 63 of p53 in the saliva of OSCC patients. This study suggests that detection of mutation in exon 4 codon 63 of p53 by PCR is a fast, reliable, accurate, and sensitive molecular method for OSCC diagnosis.


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