|Year : 2015 | Volume
| Issue : 4 | Page : 559-563
Clinical enigma: A rare case of clear cell odontogenic carcinoma
Cheshta Walia1, Rudra Prasad Chatterjee2, Sanchita Kundu2, Sudip Roy3
1 Department of Oral and Maxillofacial Pathology, Buraydah Private Dental College, Buraydah 31717, Saudi Arabia
2 Department of Oral and Maxillofacial Pathology, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India
3 Department of Orthodontics, Buraydah Private Dental College, Buraydah 31717, Saudi Arabia
|Date of Web Publication||19-Nov-2015|
Department of Oral and Maxillofacial Pathology, Buraidah Private Dental College, Buraidah 31717
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Clear cell odontogenic carcinoma is a rare, aggressive neoplasm of the jaw with only 74 reported cases. It occurs predominantly in the mandibular anterior region during fifth to seventh decades of life. Clinically it manifests as intra-bony swelling with a variable degree of pain. Microscopically, it reveals nests of cells with clear cytoplasm in connective tissue stroma arranged in different patterns. It is often misdiagnosed due to the rarity of lesion and confusing histopathology. Immunohistochemical staining plays an intricate role to uncertain the native of the clear cell to reach a confirmative diagnosis. The article aims to highlight the clinicopathologic features of clear cell odontogenic carcinoma in a middle-aged man with special emphasis on its differential diagnosis.
Keywords: Biphasic pattern, clear cell odontogenic carcinoma, clear cells
|How to cite this article:|
Walia C, Chatterjee RP, Kundu S, Roy S. Clinical enigma: A rare case of clear cell odontogenic carcinoma. Contemp Clin Dent 2015;6:559-63
| Introduction|| |
Clear cell lesions are the topic of controversy since a long time. The morphologically similar appearing clear cells are ambiguous in nature and believed to be originated from different cell lineages such as epithelial, mesenchymal, melanocytic, or hematopoietic. Clear cell odontogenic carcinoma (CCOC) is one among the rare neoplasm with only 74 reported cases in the literarure. It was first described by Hansen et al. and later by Waldron et al., in 1985, as clear cell odontogenic tumor.,, In 2005, the World Health Organization (WHO) had reclassified as CCOC owing to its high rate of recurrence, local and distant metastasis, and tumor-related deaths.
CCOC usually present with a variable degree of pain and mobility of regional teeth with or without the involvement of bone. Approximately 60% of patients show evidence of soft tissue involvement in the anterior portion of the mandible as lesion perforates bone.,
Radiographically, a poorly delineated, unilocular or multilocular radiolucent lesion with prominent bone destruction is observed. Histopathologically, CCOC may show one or more architectural patterns such as biphasic, monophasic, and ameloblastomatous.
The undetermined source of clear cells makes the diagnosis based on conventional histology nearly impossible. They require special attention to be differentiated from other clear cell lesions either by means of special stains such as mucicarmine, Congo red and periodic acid Schiff's (PAS) or immunohistochemical staining such as cytokeratin (CK), epithelial membrane antigen (EMA), S-100 protein, and vimentin. Expression of CK-19 and EMA is a consistent finding in CCOC whereas the tumor shows negativity for vimentin, S-100 protein, desmin, and smooth muscle actin.,
This article presents another unique case of CCOC with varied clinicopathological, radiological, and histopathological findings affecting the symphyseal region of the mandible in a middle-aged patient.
| Case Report|| |
A male patient aged 46 years reported to outpatient department with the chief complain of swelling in the lower front tooth region since 4–5 months. Intraorally, firm, nontendered, and exophytic lesion measuring 2.5 cm × 4 cm was observed involving mandibular anterior jaw region with exfoliation of 31, 32 and 41, 42. The alveolar sockets were unhealed with no signs of lymphadenopathy [Figure 1]. The patient's personal, family, and medical histories were noncontributory. Orthopantomogram revealed the presence of an ill-defined radiolucent destructive lesion in the mandibular anterior region [Figure 2]. Computed tomography view showed well-defined hypodense area with the complete loss of buccal cortical plate and severe thinning of the lingual cortical plate in mandibular symphysis region [Figure 3].
|Figure 2: Orthopantomogram and computed tomography scan showing bone destruction in the symphysis region|
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|Figure 3: H and E stained section showing (a) islands of clear cells in fibrous connective tissue stroma (×40), (b) clear cells mixed with columnar cells with eosinophilic cytoplasm (×100)|
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Keeping the clinical and radiological features in view, the provisional diagnosis of squamous cell carcinoma was made. The incisional biopsy was performed after obtaining informed consent from the patient, and the tissue sections were stained with hematoxylin and eosin (H/E) for microscopic evaluation.
H/E staining of tissue sections revealed the presence of islands and sheets of round or polyhedral shaped hyperchromatic clear cells with the distinct cytoplasmic membrane. These cells were surrounded by a mature fibrous stroma with variable cellularity. The tumor cells adjacent to the fibrovascular septa were cuboidal to columnar with reversed polarity of the nucleus and eosinophilic cytoplasm whereas central cells were large, round to polygonal with clear cytoplasm. There were no signs of necrosis noted in tissue sections. Mitotic figures were insignificant without any fibrous capsule at the periphery of the tumor [Figure 4].
|Figure 4: Photomicrograph showing (a) Congo red stain, (b) mucicarmine stain and (c) periodic acid-Schiff stain. Congo red and mucicarmine are negative while periodic acid-Schiff stain has taken up by cytoplasmic granules|
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Special stains such as mucicarmine, Congo red, and PAS were done to check the content of clear cells for mucin, amyloid, and glycogen deposition, respectively. Mucicarmine and Congo red were negative while PAS was strongly positive suggesting the glycogen content of clear cells [Figure 5]. CK-19 and EMA showed strong immunoreation to the tumor cells whereas S-100 protein and vimentin were nonreactive.
|Figure 5: Photomicrograph showing (a) cytokeratin, (b) epithelial membrane antigen, (c) vimentin, (d) S-100. The tumor shows strong positivity for keratin (cytokeratin) and epithelial islands (epithelial membrane antigen). The vimentin immunoreactivity is positive for fibrous tissue stroma only while S-100 was negative|
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The histopathological features confirmed the odontogenic origin of tumor and diagnosis of CCOC of the biphasic pattern was made. Further, the patient was referred to oral surgery department for surgical excision of the lesion. The excised tissue showed the similar findings as that of incisional biopsy. The postoperative recovery was uneventful, and no recurrence was observed in 3 years follow-up period.
| Discussion|| |
Clear cell variant of odontogenic tumors is rare entity of jaws. Initially, it was considered to be a benign neoplasm, but later due to its aggressive nature, spread, and recurrence, it is redesignated by the WHO as CCOC in 2005. Clear cells appear to be clear due to the presence of an intracellular accumulation of nonstaining compounds, such as glycogen, mucopolysaccharides, lipids, and mucin. The derivatives of the dental lamina or cell rests of Malassez have been suggested to be the origin of clear cells in jaws. It is believed that during late bell stage, cells of inner enamel epithelium undergo histodifferentiation to presecretory ameloblast. Electron microscope shows the presence of lysosomes, mitochondria, tonofilaments, and desmosomes. It is now known that bone morphogenetic protein (BMP-2) plays a crucial role in regulating Ms × 1 and Ms × 2 along with Dl × 2. The combined effect of Ms × 1, Ms × 2, and Dl × 2 influences Dl × 3, which normally help ameloblast cell to differentiate. Absence of these two homobox genes may be due to dysregulation of BMP-2 expression affecting the terminal differentiation of ameloblast cell. In addition to this, the genomic analysis also revealed a polypoid population of cells with DNA index of 1.93 and overall S-phase of 10.2% suggesting chromosomal alterations in cases of CCOC.
A wide age range from 14 to 89 years has been described with the peak incidence noted in the sixth decade of life. Females out numbers males with the ratio of 2:1. More than 80% of the lesions developed in the anterior portion of the mandible. The clinical presentation may vary from mild to extensive pain and bony swelling. Loosening of teeth and paresthesia is present occasionally.
The conventional radiological findings usually show poorly delineated, unilocular or multilocular radiolucent lesion that occurs with prominent bone destruction. The divergence of roots with or without root resorption is evident in few cases.
The light microscopic features of CCOC reveal three histological patterns: Biphasic, monophasic, and ameloblastomatous. The most common biphasic pattern shows two sets of cellular population arranged in sheets and islands. Center cells are clear, round to polygonal in shape mixed with another population of cuboidal to columnar cells with eosinophilic cytoplasm at the periphery. The monophasic pattern has islands of clear cells wholly. The ameloblastomatous pattern is least common, characterized by the presence of clear cells inside the follicular network., The degree of nuclear pleomorphism, hyperchromatism, and mitotic figures are variable. Encapsulation is rarely present. It has a higher tendency for the invasion to the medullary bone, muscle, and the neural tissue.
Differential diagnoses include calcifying epithelial odontogenic tumor, mucoepidermoid carcinoma, myoepithelial carcinoma, hyalinising clear cell carcinoma, epithelial-myoepithelial carcinoma, amelanotic melanoma, and metastatic renal cell carcinoma [Table 1].,,,,,,,,
We could demonstrate intracellular glycogen by means of PAS-positive, diastase-sensitive granule accumulation within the tumor cells whereas mucin and amyloid were excluded by negative mucicarmine and Congo red staining. CCOC is differentiated from calcifying epithelial odontogenic tumor due to the absence of calcifications and amyloid deposition (Congo red negative). Lack of intermediate cells, squamous differentiation, and mucin (mucicarmine negative) production excluded mucoepidermoid carcinoma. Metastatic lesion is differentiated from CCOC microscopically as later lacks the prominent sinusoidal vascularity and intramural hemorrhage that characterize metastatic renal carcinoma. Immunohistochemically, the tumor cells showed positive staining for wide spectrum CK and EMA, but negative staining for vimentin and S-100 protein. The histochemical staining profile clearly spells the odontogenic nature of neoplasm excluding the possibility of salivary gland origin.
The overall recurrence rate is as high as 55%. The treatment should aim at wide surgical resection with tumor-free margins adjunct to radiotherapy. A long-term follow-up is necessary to look for any loco-regional recurrence and distant metastasis.
| Conclusion|| |
Conspicuous nature of clear cells in CCOC poses a great challenge to a pathologist to diagnose and differentiate from other clear cell lesions. The caution should be exercised to carefully determine the source of clear cells. These type of lesions demands diagnosis based on conventional histopathology adjuvant to special staining and immunohistochemistry for optimal management considering the risk of recurrence and metastases.
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Conflicts of interest
There are no conflicts of interest.
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[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]