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Year : 2013  |  Volume : 4  |  Issue : 4  |  Page : 421-426

A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system

Professor Dr. Raad Niama Dayem BDS, MSc, PhD Conservative Dentistry, USA

Correspondence Address:
Raad Niama Dayem
6572 Parkview Dr Troy, MI 48098
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0976-237X.123015

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Objective: The objective of this study is to assess the deproteinizing effect of bromelain enzyme and compare it with neodymium-doped yttrium aluminum garnet (Nd:YAG) laser and 10% sodium hypochlorite (NaOCl) by using scanning electron microscope (SEM) and polarized microscope. Materials and Methods: A total of 60 extracted human upper premolars were selected to be given standardized buccal and lingual class V cavities. The teeth were divided into three groups each one consisted of 20 teeth. Thirty teeth were recruited for SEM study and the other 30 for polarized microscope. Group 1: Teeth were deproteinized with Nd:YAG laser, Group 2: Teeth were deproteinized with bromelain enzyme and Group 3: Teeth were deproteinized with 10% NaOCl. Results and Conclusions: Application of bromelain enzyme has led to removing collagen network and significantly decreased the global leakage scores of the adhesive system.

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